|29 (Research article)
|Malmström, Lars; Malmström, Johan; Selevsek, Nathalie; Rosenberger, George; Aebersold, Ruedi
|Automated workflow for large-scale Selected Reaction Monitoring experiments.
|J Proteome Res (2012) 11 1644-53
|27 citations (journal impact: 5.06)
|Targeted proteomics allows researchers to study proteins of interest without being drowned in data from other less interesting proteins or from redundant of uninformative peptides. While the technique is mostly used for smaller focused studies there are several reasons to conduct larger targeted experiments. Automated highly robust software becomes more important in such experiments. In addition larger experiments are carried out over longer periods of time requiring strategies to handle the sometimes large shift in retention time often observed. We present a complete proof-of-principle software stack that automates most aspects of selected reaction monitoring workflows a targeted proteomics technology. The software allows experiments to be easily designed and carried out. The steps automated are the generation of assay generation of mass spectrometry driver files and methods files the import and analysis of the data. All data is normalized to a common retention time scale and the data is then scored using a novel score model and the error is subsequently estimated. We also show that Selected Reaction Monitoring can be used for label-free quantification. All data generated is stored in a relational database and the growing resource further facilitates the design of new experiments. We apply the technology to a large-scale experiment studying how S. pyogenes remodels its proteome under stimulation of human plasma.
|This paper scribes an automated system to carry out and analyze data from selected reaction monitoring mass spectrometry experiments.