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Identification of secreted glycoproteins of human prostate and bladder stromal cells by comparative quantitative proteomics.

Type Information
Nr 18 (Research article)
Authors Goo, Young; Liu, Alvin; Ryu, Soyoung; Shaffer, Scott; Malmström, Lars; Page, Laura; Nguyen, Liem; Doneanu, Catalin; Goodlett, David
Title Identification of secreted glycoproteins of human prostate and bladder stromal cells by comparative quantitative proteomics.
Journal Prostate (2009) 69 49-61
DOI 10.1002/pros.20853
Citations 28 citations (journal impact: 3.08)
Abstract BACKGROUND Functional development of the prostate is governed by stromal mesenchyme induction and epithelial response. Stromalepithelial signaling can be mediated through direct cell-cell contact and diffusible factors and their cell surface receptors. These inducers are likely secreted or membrane-associated extracellular proteins. Given the importance of intercellular communication it is possible that diseases like cancer could arise from a loss of this communication. One approach to gain a molecular understanding of stromal cells is to identify as a first step secreted stromal signaling factors. We proposed to do this by comparative analysis between bladder and prostate. METHODS Secreted proteins were identified from cultured normal prostate and bladder stromal mesenchyme cells by glycopeptide-capture method followed by mass spectrometry. Differences in protein abundance between prostate and bladder were quantified from calculated peptide ion current area PICA followed by Western validation. Functional and pathway analyses of the proteins were carried out by Gene Ontology GO and Teranode software. RESULTS This analysis produced a list of 116 prostate and 84 bladder secreted glycoproteins with ProteinProphet probability scores 0.9. Stromal proteins upregulated in the prostate include cathepsin L follistatin-related protein neuroendocrine convertase tumor necrosis factor receptor and others that are known to be involved in signal transduction extracellular matrix interaction differentiation and transport. CONCLUSIONS We have identified a number of potential proteins for stromal signaling and bladder or prostate differentiation program. The prostate stromalepithelial signaling may be accomplished through activation of the ECM-receptor interaction complement and coagulation cascades focal adhesion and cell adhesion pathways. Prostate c 2008 Wiley-Liss Inc.